ANTIBIOTIC SENSITIVITY PATTERN OF YERSINIA ENTEROCOLITICA ISOLATED FROM MILK AND DAIRY PRODUCTS* T.R.Pugazhenthi 1, A. Elango2 , C. Naresh Kumar3 , B. Dhanalakshmi4 and A. Bharathidhasan5
Department of Dairy Science, Madras Veterinary College, Chennai – 600 007. ABSTRACT
From a total of 2431 samples of milk and milk products analyzed at
Chennai, twenty isolates of bacteria were identified as Yersinia species by theconventional biochemical method which includes nine Yersinia enterocolitica(0.37%), six Y. frederiksenii (0.25%) and five Y. kristensenii (0.21%). The sensitivity pattern of six antimicrobial agents viz. ampicillin, chloramphenicol,ciprofloxacin, gentamicin, penicillin and tetracycline were tested against nineisolates of Y. enterocolitica. The invitro antibiotic sensitivity testing showedthat ciprofloxacin (5 mcg) and tetracycline (30 mcg) were the most effectiveantibiotics against Y. enterocolitica, whereas, penicillin (10 units) was the mostineffective one. Hence, analyzing the prevalence and antibiotic sensitivity patternof Y. enterocolitica isolates from dairy products is essential. Key words: Milk - Yersinia enterocolitica - antibiotic resistance
Associate Professor, Dept. of Livestock Products Technology, Instructional Livestock FarmComplex Campus, Veterinary College and Research Institute, Tirunelveli-627 358. E-mail : [email protected]
Professor and Head, Department of Dairy Science, Veterinary College and ResearchInstitute, Namakkal – 1.
Assistant Professor, Department of Animal Nutrition
* Part of the Ph.D. thesis of the first and corresponding author submitted to TANUVAS, Chennai. Tamilnadu J. Veterinary & Animal Sciences 9 (2) 141 - 145, March - April 2013
Antibiotic sensitivity pattern of yersinla enterocolitica
emergence of microorganisms resistant toantibiotics currently used in human life.
of frequent occurrence in India, because of their
MATERIALS AND METHODS
perishable nature and the tropical environmentalconditions prevailing in India. The genus Yersinia
Isolation and identification of Yersinia
comprises three well known food borne human
species from aseptically collected 2431 milk and
pathogens: Yersinia enterocolitica,
milk products samples including 1410 raw milk,
Y. pseudotuberculosis and Y. pestis. Among
185 pasteurized milk, 308 buttermilk, 113 khoa,
these three, Y. enterocolitica is important milk
205 ice cream and 210 paneer from various
borne pathogen and its presence in milk is of great
zones of Chennai during different seasons were
public health importance by causing yersiniosis,
carried out as per the procedure of standard
an emerging disease (Bercovier and Mollaret,
methods for examination of dairy products
1984), which is characterized by gastroenteritis
(Robert, 1992 and Bottone et al., 2005). Nine
with diarrhoea, vomiting, fever and abdominal
isolates of Y. enterocolitica were identified
based on various biochemical tests as well asmolecular methods and were subjected to
ubiquitous microorganism and majority of theisolates recovered from food are non pathogenic,
it is important to determine the control measures
Y. enterocolitica isolates (200 µl each) were
on the growth of Yersinia enterocolitica by
spread-plated onto Mueller Hinton agar (Hi
antibiotics. Antimicrobials are used in food
Media) aseptically. Antibiotic sensitivity pattern
animals as therapeutic agents and as growth
was determined by the disc diffusion method
promoters. The use of effective drugs has been
using commercially available discs ((M/s. Hi
essential to guarantee the high indices of
Media Laboratories Pvt. Ltd., Mumbai, India)
productivity reached in the last decades,
with the following antibiotics with concentration
propitiating a reduction of mortality and
noted against each: Ampicillin (A) – 10 mcg,
morbidity and the maintenance of animal well–
Chloramphenicol (C) – 30 mcg, Ciprofloxacin
being. However, the indiscriminate use of
(Cf) – 5 mcg, Gentamicin (G) – 10 mcg, Penicillin
antimicrobials can lead to the selection of
(P) – 10 units and Tetracycline (T) – 30 mcg.
resistant bacteria in food animals that could
Antibiotic discs were placed at equidistance in
each plate and incubated at 250 C for 24 hrs.
serious public health problem (McEwen and
The inhibition zone (including diameter of disc)
was measured and interpreted as per the chartsof Kushal and Anand, 2001. Isolates were
considered resistant if no zone of inhibition could
pathogens is an important concern to the world
be seen around the disc. The statistical analysis
today. Use of antibiotics to prevent diseases
was carried out as per the procedure adopted
such as mastitis in cows may contribute to the
Tamilnadu J. Veterinary & Animal Sciences 9 (2) 141 - 145, March - April 2013RESULTS AND DISCUSSION
These findings are in agreement with thestudies of Pham et al. (1991) and Lal et al.
(2003) who reported that Y.enterocolitica was
assumed as presumptive Yersinia speciesfrom a
sensitive to chloramphenicol, ciprofloxacin,
total of 2431 samples of milk and milk products
gentamicin, tetracycline and trimethoprim and
collected and analysed from various sources in
resistant to ampicillin and penicillin. Statistical
and around Chennai. Through various biochemical
analysis shows that there was highly significant
tests, 20 isolates were identified and confirmed
difference (P d” 0.01) with regard to antibiotic
as Yersinia sp. which includes nine Y.
sensitivity pattern on Y. enterocolitica. enterocolitica (0.37%), six Y. frederiksenii(0.25%) and five Y. kristensenii (0.21%). REFERENCES
The same was also confirmed by molecularmethods like Polymerase Chain Reaction (PCR)
Bercovier, H. and Mollaret, H. H. (1984).
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Antibiotic sensitivity pattern of yersinla enterocolitica
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and IBH Publishing Company, NewDelhi, India. Tamilnadu J. Veterinary & Animal Sciences 9 (2) 141 - 145, March - April 2013Antibiotic resistance profile of Yersinia enterocolitica Y.enterocolitica Particulars of antibiotic disc isolates Ampicillin Ciprofloxacin Gentamicin Penicillin Tetracycline Phenicol (10 units) (30 mcg) Animal Sciences 9 (2) 141 - 145, Mar
χ2 = 64. 67**
R – Resistant S - Sensitive I - Intermediate
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